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Journal: Molecular Systems Biology
Article Title: The genotype‐phenotype landscape of an allosteric protein
doi: 10.15252/msb.202010179
Figure Lengend Snippet:
Article Snippet: Add 250 μl lysis buffer (200 mmol/l NaOH, 10 g/l SDS) to each sample and mix by shaking the plate at 90 rpm for 2 min. Add 350 μl cold (4°C)
Techniques: Recombinant, Plasmid Preparation, Sequencing, Amplification, Mutagenesis, Neutralization, Binding Assay, Membrane, Software, Purification, Gel Extraction, Flow Cytometry